Table 1.
Choice of targets for validation by RT-qPCR from OpenArray analysis
| miRNA | Significance of fold change in CM vs. NI MV | |
|---|---|---|
| OpenArray | RT-qPCR | |
| hsa-miR-328 | − 2.5* ± 0.93 | Not testeda |
| hsa-miR-335* | − 3.0* ± 1.13 | Not testeda |
| mmu-miR-16* | 2.8** ± 0.65 | Not testeda |
| mmu-miR-21* | 5.0** ± 0.88 | Not testeda |
| mmu-miR-297a* | 5.8* ± 1.60 | Not testeda |
| mmu-miR-685 | 3.0* ± 1.00 | Not testeda |
| mmu-miR-1949 | 5.0* ± 1.69 | Not testeda |
| hsa-miR-590-5p | Unique to NI | Not validatedb |
| rno-miR-450 | Unique to CM | Not validatedb |
| mmu-miR-10b | 2.7* ± 0.85 | Not validatedb |
| hsa-miR-146a | 3.2** ± 0.68 | 7.2* ± 2.74 |
| hsa-miR-150 | 1.8* ± 0.64 | 2.7 (ns) ± 2.26 |
| hsa-miR-205 | 2.3* ± 0.75 | − 0.5 (ns) ± 1.89 |
| hsa-miR-486 | 2.3*** ± 0.18 | 4.7 (ns) ± 1.45 |
| mmu-miR-193b | − 2.7** ± 0.62 | − 7.5* ± 0 62 |
| mmu-miR-215 | 2.1* ± 0.55 | 4.6 (ns) ± 99.39c |
| mmu-miR-467a | − 2.0* ± 0.69 | − 5.6 (ns) ± 0.96 |
The list of significantly differentially expressed miRNA in CM vs NI MV from the OpenArray analysis was compared with the results obtained by RT-qPCR analysis. Results presented as relative quantification ± SEM
P: * = 0.05–0.01, ** = 0.01–0.0001, *** ≤ 0.0001
amiRNA not validated by RT-qPCR due to lack of relevance to this study
bmiRNA not validated despite being tested by RT-qPCR due to technical issues
cLarge SEM variation due to outliers in NI and CM MV groups