Table I.
Purification of dHG-6-OMT from cotton stele tissue infected with V. dahlia
| Purification Stepa | Volume | Protein | Total Activity | Specific Activity | Purification | Recovery |
|---|---|---|---|---|---|---|
| mL | mg | pkat | pkat mg−1 protein | -fold | % | |
| Crude extract | 304 | 86.8 | 298.7 | 3.44 | 1 | 100 |
| Q-Sepharose, FF | 30 | 3.504 | 73.7 | 21.0 | 6.1 | 25 |
| Ultragel AcA34 | 31.5 | 0.797 | 46.4 | 58.2 | 16.9 | 16 |
| 3H4MPEA-Sepharose | 16 | 0.402 | 23.8 | 59.4 | 17.3 | 8 |
| 3,4DMPEA-Sepharose | 4 | 0.0095 | 5.75 | 608 | 177 | 1.9 |
| 2′,5′-ADP-Sepharose | 3 | 0.0014 | 3.95 | 2,926 | 851 | 1.3 |
a
3H4MPEA-Sepharose: 3-Hydroxy-4-methoxyphenethylamine-Sepharose; 3,4DMPEA-Sepharose: 3,4-dimethoxyphenethylamine-Sepharose; 2′,5′-ADP-Sepharose: adenosine 2′,5′-diphosphate-Sepharose 4B.