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. 2018 May 4;9:892. doi: 10.3389/fmicb.2018.00892

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Copyright © 2018 Peng, Liu, Zhao, Wang and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Determination of the optimal number of reference genes for normalization. Pairwise variation (V_n/V_n+1) analysis between the normalization factors (NF_n and NF_n+1) was performed by geNorm to determine the optimal number of reference genes.