Table 1.
Gene descriptions and primer sequences used for RT-qPCR.
| Gene Symbol | Annotation | LinRegPCR Efficiency | Sequence (5′-3′) | Tm(°C) | References |
|---|---|---|---|---|---|
| ldhD | D-Lactate dehydrogenase | 1.845 | F-GCCGCAGTAAAGAACTTGATG | 58.3 | Margalef-Català et al., 2016 |
| R-TGCCGACAACACCAACTGTTT | |||||
| dnaG | DNA primase | 2.041 | F-TGTGGACGGAGTGGCAATGT | 62 | Desroche et al., 2005 |
| R-CGGTATTTTCTGTATATTTACTATCG | |||||
| gyrA | DNA Gyrase subunit A | 1.930 | F-CGCCCGACAAACCGCATAAA | 62 | Desroche et al., 2005 |
| R-CAAGGACTCATAGATTGCCGAA | |||||
| gyrB | DNA Gyrase subunit B | 1.875 | F- GGTTGAGGCTGGTCGAGTGTAT | 62.3 | This study |
| R- GCATCCATCTCACCAAGTCCCT | |||||
| rrs | 16S ribosomal RNA | 1.847 | F-ATGGTCGTCGTCAGCTCGTG | 60.3 | This study |
| R- TGTGTTGCCCAGGTCATAAGG | |||||
| ddlA | D-Alanyl-alanine synthetase A | 1.994 | F- ATGGCAGTGGATGGTTTGACT | 58.3 | This study |
| R-TAGTGTATTAGGCTCGCTTAGGAA | |||||
| rpoA | RNA polymerase subunit α | 2.066 | F- TGCTGGGAAGAAAGAAATGATG | 56.3 | This study |
| R-AGTTAAACGAACGAACCGAAAG | |||||
| proC | Pyrroline-5-carboxylate reductase | 1.876 | F-CTGCTTGCTGATTGCGATTT | 58 | This study |
| R-CCGTTAGTTCTTTAAGGCTTGTTG | |||||
| dpoIII | DNA polymerase III, alpha subunit | 1.804 | F-GCAGTGAAGGGACGCTTAAACG | 62.3 | Costantini et al., 2011 |
| R-ACCCAATCGCCTCGACATCATC | |||||
| hsp18 | Heat shock protein Lo18 | 1.925 | F-TGTGGACGGAGTGGCAATGT | 60.3 | Beltramo et al., 2006 |
| R-CGGTATTTTCTGTATATTTACTATCG |