Table 2.

Gene expression stability ranked by geNorm, NormFinder, and BestKeeper in all samples and 8, 12, and 16% ethanol shock treatment.

Group	Overall rank	Gene	geNorm	M-value	Normfinder	Stability value	Bestkeeper	SD (± Ct)	CV(% Ct)
Total	1	dnaG	2	0.448	1	0.129	1	0.518	2.35
	2	dopIII	1	0.431	6	0.260	2	0.623	2.703
	3	gyrA	3	0.482	2	0.141	4	0.719	3.36
	4	gyrB	4	0.673	3	0.157	5	0.900	3.73
	5	proC	5	0.773	5	0.241	6	0.906	3.76
	6	rrs	7	0.879	4	0.166	7	0.543	6.34
	7	ddlA	9	0.999	9	0.547	3	0.699	3.14
	8	ldhD	6	0.83	8	0.510	9	1.196	6.13
	9	rpoA	8	0.917	7	0.365	8	1.151	5.87
8% ethanol ST	1	dpoIII	1	0.204	1	0.270	2	0.800	3.38
	2	dnaG	2	0.225	2	0.272	3	0.800	3.54
	3	gyrA	3	0.251	4	0.352	5	0.980	4.54
	4	ddlA	9	1.022	9	1.036	1	0.710	3.14
	5	rrs	7	0.802	3	0.349	4	0.310	3.78
	6	gyrB	6	0.735	5	0.497	6	1.140	4.56
	7	rpoA	4	0.863	6	0.527	8	1.180	5.92
	8	ldhD	5	0.573	8	0.863	9	1.210	6.04
	9	proC	8	0.632	7	0.747	7	1.390	5.57
12% ethanol ST	1	gyrB	4	0.503	3	0.296	1	0.270	1.15
	2	gyrA	3	0.411	1	0.130	4	0.560	2.65
	3	rrs	1	0.32	4	0.434	7	0.510	5.97
	4	proC	2	0.334	6	0.479	3	0.590	2.5
	5	dopIII	5	0.625	5	0.467	2	0.510	2.27
	6	dnaG	6	0.673	2	0.150	6	0.660	3.05
	7	ddlA	7	0.74	7	0.768	5	0.670	3.03
	8	ldhD	8	0.882	9	0.880	8	1.230	6.3
	9	rpoA	9	0.975	8	0.794	9	1.320	6.71
16% ethanol ST	1	proC	1	0.216	1	0.025	2	0.470	2
	2	gyrB	2	0.242	1	0.025	3	0.520	2.15
	3	dnaG	5	0.504	4	0.248	1	0.330	1.51
	4	rrs	3	0.279	3	0.081	9	0.620	7.09
	5	gyrA	4	0.391	5	0.250	5	0.620	2.88
	6	dopIII	7	0.631	7	0.615	6	0.700	3.03
	7	ddlA	9	0.881	9	1.038	4	0.610	2.77
	8	rpoA	8	0.727	6	0.575	7	0.700	3.68
	9	ldhD	6	0.572	8	0.907	8	0.760	4.02

SD (± Ct): standard deviation of the Ct; CV (%Ct), coefficient of variance expressed as a percentage of the Ct level; ST, shock treatment.