Figure 1.

RNA gel blot with total (A) or poly(A⁺) RNA (B) from various tissues as shown. Amounts loaded are as follows: A, 10 μg of total RNA; B, 3 μg of poly(A⁺) RNA from kernel and 12 μg of total RNA from pollen; C, 20 μg of total RNA from suspension-cultured cells. The blots were initially hybridized with an Ivr1 (exons 3–D4 region) ³²P-labeled probe and subsequently hybridized with a probe consisting of the entire coding region of Incw2 or Incw1. −S, 48 h Suc-depeletion; +S and +M, 12-h additions of Suc or mannitol, respectively.