Figure 3.

Rotofor fractionation of invertase activity in supernatant and pellet fractions from 12-DAP kernels. Protein extracts were subjected to IEF in solution, using a Rotofor cell and pH 3.0 to 10.0 ampholytes. The activity peaks from soluble (Sup) and pellet fractions were pooled and concentrated as described in “Materials and Methods.” For the SDS-PAGE immunoblot, equal amounts of total activity were loaded at 12 μmol/h. Numbers above activity peaks correspond to the measured pH for that fraction. Antisera dilutions and detection are described in “Materials and Methods.”