Figure 4.

Rotofor fractionation of invertase activity in the supernatant fraction from pollen. The pollen protein extract was prepared as described in “Materials and Methods” and subjected to IEF in the Rotofor cell using pH 3.0 to 10.0 ampholytes. For the SDS-PAGE immunoblot, fraction 7 was concentrated and 1 μmol Glc/h was loaded. Protein (15 μg) from kernel extract was loaded as a control. Antisera dilutions and detection are described in “Materials and Methods.” The blot was first probed with anti-IVR1 antisera, stripped, and reprobed with anti-INCW1 antisera.