Table II.

Invertase activity from various sugar treatments of maize cell-suspension cultures

Treatment	Fraction	nmol Glc μg−1/h−1	Percentage of Initial
0 h	Supernatant	25	100
	Pellet	64	100
48 h − Suc	Supernatant	6.7	27
	Pellet	12	18
12 h + Suc	Supernatant	11	44
	Pellet	23	36
12 h + mannitol	Supernatant	5.5	22
	Pellet	10	16

The results of Suc starvation and subsequent addition of Suc or mannitol to suspension-cultured cells. Seven-day-old cultures (0 h) were washed with Suc-free medium and grown without Suc for 48 h prior to the addition of 2% Suc or mannitol for 12 h. All enzymatic measurements were in triplicate, with sd values less than 10%.