Figure 1.

Characteristics of the T Cell Hybridomas Specific to Several T7S Substrates

(A–D) Specificity and sensitivity of T cell hybridomas specific to various substrates of ESX-1 (A), ESX-3 (B), ESX-5 (C), or Tat (D) systems, as determined by their co-culture with DCs, loaded with various concentrations of peptides harboring MHC class II epitopes or negative controls.

(E–H) Capacity of these T cell hybridomas, specific to substrates secreted by ESX-1 (E), ESX-3 (F), ESX-5 (G), or Tat (H) secretion systems, to recognize DCs infected with WT Mtb H37Rv, BCG, or appropriate Mtb mutants. Shown are concentrations of IL-2 in the co-culture supernatants at 24 hr after T cell addition. Error bars represent the SD of biological co-culture duplicates. Results are representative of 2 experiments.

See also Figures S1 and S2 and Table S1.