Table 4.
Prevalence of Aminoglycoside-Modifying Enzyme Genes and Correlation with Expected and Observed Antibiograms in 139 Klebsiella pneumoniae Producing Extended Spectrum β-Lactamase
| AME gene | No. of isolates (%) | Expected resistance phenotype | Observed resistance phenotype (No. of isolates) EUCAST |
|---|---|---|---|
| aac(6′)-Ib | 28 (20.1) | Ak, K, Nt, To | As expected (0) |
| K, Nt, To (14) | |||
| To (3) | |||
| K, To (3) | |||
| K, Nt (2) | |||
| Gm, K, Nt, To (1) | |||
| K (1) | |||
| Susceptible (4) | |||
| aac(3)-Ia | 9 (6.5) | Gm | As expected (2) |
| Susceptible (7) | |||
| aph(3′)-Ia | 2 (1.4) | K, Nm | As expected (2) |
| aac(3)-IIa | 31 (22.3) | Gm, Nt, To | As expected (13) |
| Gm, To (2) | |||
| Gm, Nt (2) | |||
| Gm (14) | |||
| aac(3)-IVa | 2 (1.4) | Gm, Nt, To | As expected (2) |
| aac(6′)-Ib, aac(3)-IIa | 28 (20.1) | Ak, Gm, K, Nt, To | As expected (1) |
| Gm, K, Nt, To (21) | |||
| Gm, K, Nm, Nt, To (1) | |||
| Gm, Nt, To (3) | |||
| Gm, To (1) | |||
| Gm, Nt (1) | |||
| aph(3′)-IIa, aac(3")-IVa | 7 (5.0) | Gm, K, Nm, Nt, To | As expected (7) |
| aac(6′)-Ib, aph(3′)-Ia | 2 (1.4) | Ak, K, Nm, Nt, To | Gm, K, Nt, To (1) |
| K (1) | |||
| aac(6′)-Ib, aph(3′)-IIa | 1 (0.7) | Ak, K, Nm, Nt, To | K, Nm, Nt, To (1) |
| aac(6′)-Ib, ant(2")-Ia | 1 (0.7) | Ak, Gm, K, Nt, To | K, Nt, To (1) |
| ant(2")-Ia, aac(3)-IVa | 1 (0.7) | Gm, K, Nt, To | As expected (1) |
| aph(3′)-Ia, aac(3)-IVa | 1 (0.7) | Gm, K, Nm, Nt, To | As expected (1) |
| aac(6′)-Ib, ant(2")-Ia, aph(3′)-Ia | 1 (0.7) | Ak, Gm, K, Nm, Nt, To | Gm, K, Nm, Nt, T (1) |
| aac(6′)-Ib, aac(3)-IIa, ant(2")-Ia | 1 (0.7) | Ak, Gm, K, Nt, To | As expected (1) |
| None | 24 (17.26) | Susceptible | As expected (24) |
| Total No. of isolates | 139 (100) |