Figure 1.

Depletion of diverse populations of CD32-expressing CD4 T cells by negative bead selection due to the expression of non-T cell markers. (A) The frequency of CD32⁺CD3⁺CD4⁺ T cells when measured in peripheral blood mononuclear cells (PBMCs), or in CD4 T cells purified by negative bead selection, with and without Fc block prior. (B) Representative gating of data shown in panel (A). (C) Gating of three CD32-expressing populations based on differing levels of expression and CD14: CD32^low, CD32⁺CD14⁺, and CD32^high. (D) Heat-map showing the percentage expression of markers used in CD4 negative bead isolation kits on subsets of CD3⁺CD4⁺ T cells [defined in panel (C)]. Expression was measured by flow cytometry, and values are shown as the mean of three individual donors. (E) Percentage expression of CD11b, CD19, CD36, and CD20 on CD3⁺CD4⁺ subpopulations as shown in panel (D). Bar is shown at the mean.