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. 2018 May 4;9:928. doi: 10.3389/fimmu.2018.00928

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Copyright © 2018 Martin, Pace, Thornhill, Phetsouphanh, Meyerowitz, Gossez, Brown, Olejniczak, Lwanga, Ramjee, Kaleebu, Porter, Willberg, Klenerman, Nwokolo, Fox, Fidler and Frater.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Characterization of T cell receptor and immunoglobulin (Ig) expression on CD32^highCD3⁺CD4⁺ T cells. (A) Expression of TCRαβ on CD32-expressing CD3⁺CD4⁺ T cell populations was measured by flow cytometry and is shown here as median fluorescence intensity. Bar is shown at the mean. (B) Histogram showing TCRαβ staining on CD32-expressing CD3⁺CD4⁺ T cell populations of one sample shown in panel (A). Fluorescence minus one (FMO) controls are shown as dashed lines and individual populations as solid lines. (C) Percentage expression of Ig was measured on CD3⁺CD4⁺CD32^highCD20⁺ cells. Expression of IgD, IgG, and IgM is shown. Expression was measured by flow cytometry, and bar is shown at the mean. (D) Representative staining of data shown in panel (C).