FIG. 3.
In vivo fluorescence signals. Representative images of the red and green fluorescent signals demonstrate the relative intensity of GFP and tdT from in vivo imaging. After removing background tissue and fur autofluorescence, minimal fluorescence was present in wild-type animals in (A) the green fluorescent channel or (B) the red channel. In the ears, feet, and tail, (C) the GFP signal was observed to be significantly weaker (1.67 × 108 ± 1.22 × 107 photons/sec/cm2) than (D) the tdT signal (2.65 × 108 ± 3.35 × 107 photons/sec/cm2, p = 0.020).