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. 2018 Apr 27;9(5):2418–2435. doi: 10.1364/BOE.9.002418

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Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article's title, journal citation, and DOI.

Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article's title, journal citation, and DOI.

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Fig. 3 — Timing of fluorescence and brightfield camera (BF) exposures (grey boxes), and their associated pulsed illumination (blue and red boxes). By laser-illuminating the sample only for short intervals at the end and beginning of successive fluorescence camera exposures, we were able to sample the flow with better temporal resolution than that implied by the maximum framerate of the camera. Furthermore, the BF images used for heart synchronization may be contaminated by stray laser/fluorescence light leaking through the BF filters; this cross-talk can be avoided by sequencing the BF camera exposures to avoid the laser pulses, as shown here.