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. 2018 Apr 27;9(5):2418–2435. doi: 10.1364/BOE.9.002418

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Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article's title, journal citation, and DOI.

Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article's title, journal citation, and DOI.

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Fig. 5 — SPIM-μPIV analysis results of FEP tube experiments in locations of peak flow, from analysis of fluorescence imagery. Top row - 0.5 μl/min nominal flow rate beads, bottom row - 1 μl/min beads. Shaded area represents tube walls. The profiles of the u-components of velocity in xy (a,d) have very similar shapes for all experiments (semi-minor axis ranges ≈26.3 – 26.5 μm). Similarly, the xz planes (b,e) show similar profile shape (semi-major axis ranges ≈25.9 – 25.8 μm). This confirms that there is no significant anomalous bias to the flow profile recovered in the xz plane, thus validating our use of SPIM-μPIV for 3D flow mapping. (c,f) show colormaps of the velocity magnitude in the yz plane, confirming that to a good approximation the reconstructed flow is circularly symmetric and Poiseuille-like. Dashes show a diameter of 50 μm.