Table 1.

Main experimental and PIV analysis parameters summarized for both the phantom validation experiment and the zebrafish heart. Effective single pixel size was 0.3225 μm (at 20× magnification). The number of frame pairs is approximate, as it varied slightly from one plane to another due to our acquisition code. The variation was greater for the heart data, however, phase bins had around 50 frame pairs. (*) days post fertilization.

Parameter	Beads in 50 μm ø FEP, flow rate 0.5 \ 1.0 μl/min	fRBCs in the ∼3 dpf* Zebrafish heart
Laser pulse length, ms	0.02 \ 0.01	1
Inter-pulse time, ms	0.3 \ 0.5	1.5
z-step, μm	2	8
Camera pixel binning	1 × 1	2 × 2
Frame pairs per z-step	∼250 \ 150	∼ 50
Small IW (LxH), px	32 × 12	24 × 24
Big IW (LxH), px	128 × 16	72 × 48
Peak-to-peak threshold	1.10	1.07
Mask around peak, px	7 × 7	7 × 7