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. 2017 Dec 9;73(6):711–719. doi: 10.1093/gerona/glx242

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© The Author(s) 2017. Published by Oxford University Press on behalf of The Gerontological Society of America.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Effect of inhibition of Cav-3 and PKA on I_Ca density in 3- and 24-month myocytes. (A) Mean I_Ca density at 0 mV in the absence (−) and presence (+) of C3SD peptide in wild-type (WT) and Cav-3OE myocytes for myocytes from 3-month-old mice (two-way ANOVA: C3SD ns, genotype ns, interaction p < .001). (B) Corresponding data for 24-month-old mice (two-way ANOVA: C3SD ns, genotype ns, interaction ns). **p < .01 and ***p < .001 Bonferroni corrected post-hoc test. White text on bars in A and B represent sample sizes (n/N). (C) Mean I_Ca density-voltage relationships recorded in the presence of H-89 from intact 3-month WT (black circles) and Cav-3OE myocytes (gray squares). Two-way repeated measures analysis of variance (RM ANOVA): voltage p < .001, genotype p < .05, interaction p < .001. (D) Corresponding data for 24-month myocytes. Two-way RM ANOVA: voltage p < .001, genotype ns, interaction p < .01. (E and F) Data presented in C and D, rearranged to compare within (E) WT (two-way RM ANOVA: voltage p < .001, age p < .001, interaction p < .001), or (F) Cav-3OE (two-way RM ANOVA: voltage p < .001, age ns, interaction ns) mice. (G, H) Effect of PKA inhibition on mean I_Ca density at 0 mV in the absence (−, from Figure 2) and presence (+) of H-89 measured in intact or DT cells from 3-month and 24-month WT (G) and Cav-3OE (H) mice. Sample sizes for intact cells/hearts in control solution and in the presence of H-89 are provided in Figures 2 and 3, respectively. For DT myocytes, n/N were: WT 3 month = 8/3; 24 months = 6/3; Cav-3OE 3 months = 7/2; 24 months = 7/2). *p < .05, **p < .01, and ***p < .001, Bonferroni corrected post-hoc test. (I) Calculated I_Ca density at 0 mV for the whole cell, surface, or t-tubular membranes in WT myocytes in the presence of H-89. (J) Corresponding data for Cav-3OE myocytes. *p < .05, **p < .01, and ***p < .001, Student’s t test.