Table 1.
List of yeast deletion strains that suppress GR100 toxicity.
| GR100 suppressors | Systematic name | PR50 suppressor | FUS suppressor | TDP-43 suppressor | Function | Human ortholog(s) |
|---|---|---|---|---|---|---|
| Ribosomal small subunit biogenesis (16/133, P = 3.323e–4) | ||||||
| rps0aΔ | YGR214W | ribosomal 40S subunit protein; rRNA maturation | RPSA | |||
| rps1bΔ | YML063W | ribosomal 40S subunit protein | RPS3A | |||
| rps6aΔ | YPL090C | rps6bΔ | ribosomal 40S subunit protein | RPS6 | ||
| rps8aΔ | YBL072C | yes | ribosomal 40S subunit protein | RPS8 | ||
| rps11aΔ | YDR025W | ribosomal 40S subunit protein | RPS11 | |||
| rps11bΔ | YBR048W | ribosomal 40S subunit protein | RPS11 | |||
| rps16bΔ | YDL083C | ribosomal 40S subunit protein | RPS16 | |||
| rps18aΔ | YDR450W | ribosomal 40S subunit protein | RPS18 | |||
| rps24aΔ | YER074W | ribosomal 40S subunit protein | RPS24 | |||
| sac3Δ | YDR159W | ribosome biogenesis; mRNA export | SAC3D1/MCM3AP | |||
| nsr1Δ | YGR159C | yes | yes | pre-rRNA processing; ribosome biogenesis | ||
| ltv1Δ | YKL143W | yes | Ribosomal small subunit export | LTV1 | ||
| hcr1Δ | YLR192C | pre-rRNA processing; translation initiation | EIF3J | |||
| tsr2Δ | YLR435W | yes | potential role in pre-rRNA processing | TSR2 | ||
| bud21Δ | YOR078W | part of the ribosomal small subunit processosome | ||||
| bud22Δ | YMR014W | rRNA maturation; ribosome biogenesis | SRFBP1 | |||
| Additional ribosomal proteins and ribosome-associated processes (23/133) | ||||||
| rpl12aΔ | YEL054C | ribosomal 60S subunit protein | RPL12 | |||
| rpl19bΔ | YBL027W | yes | ribosomal 60S subunit protein | RPL19 | ||
| rpl21bΔ | YPL079W | ribosomal 60S subunit protein | RPL21 | |||
| rpl34aΔ | YER056C-A | ribosomal 60S subunit protein | RPL34 | |||
| rpl37aΔ | YLR185W | ribosomal 60S subunit protein; pre-rRNA processing | RPL37 | |||
| rpl38Δ | YLR325C | ribosomal 60S subunit protein | RPL38 | |||
| rps29aΔ | YLR388W | ribosomal 40S subunit protein | RPS29 | |||
| rpp1bΔ | YDL130W | component of the ribosomal stalk | RPLP1 | |||
| rpp2bΔ | YDR382W | yes | component of the ribosomal stalk | RPLP2 | ||
| cgr1Δ | YGL029W | pre-rRNA processing; nucleolar integrity | CCDC86 | |||
| hpm1Δ | YIL110W | methyltransferase; modification of ribosomal protein | METTL18 | |||
| jjj1Δ | YNL227C | ribosome biogenesis | ||||
| kap120Δ | YPL125W | karyopherin; nuclear import of ribosomal maturation factor Rpf1p | IPO11 | |||
| kns1Δ | YLL019C | serine/threonine kinase; ribosome and tRNA biogenesis; rRNA transcription | CLK1-4 | |||
| nop12Δ | YOL041C | pre-rRNA processing; ribosome biogenesis | HNRNPD/DL/A0/AB | |||
| nop16Δ | YER002W | yes | ribosome biogenesis | NOP16 | ||
| rrp8Δ | YDR083W | methyltransferase; modification of ribosomal protein; pre-rRNA processing | RRP8 | |||
| stm1Δ | YLR150W | translation and ribosome preservation during nutrient stress; binds G-quadruplexes | SERBP1, HABP4 | |||
| tif4631Δ | YGR162W | ribosome biogenesis; translation initiation | EIF4G | |||
| syh1Δ | YPL105C | unknown function, but associates with nuclear pore and ribosomes | GIGYF1/2 | |||
| tma19Δ | YKL056C | associates with ribosomes | TPT1, 1P8 | |||
| ygl088wΔ | YGL088W | yes | unknown function, but partially overlaps with a snoRNA | |||
| yor309cΔ | YOR309C | yes | dubious open reading frame (ORF), but partially overlaps with NOP58 | |||
| RNA-related processes (15/133) | ||||||
| caf120Δ | YNL278W | part of a transcriptional regulatory complex; mRNA initiation, elongation, degradation | PAK2 | |||
| cgi121Δ | YML036W | yes | part of a tRNA modification complex | TPRKB | ||
| ebs1Δ | YDR206W | nonsense mediated decay; translation inhibition | SMG5/6/7 | |||
| gim3Δ | YNL153C | part of a prefoldin complex; transcriptional elongation | PFDN4 | |||
| gis2Δ | YNL255C | yes | activation of translation of IRES-containing mRNAs | |||
| lrp1Δ | YHR081W | RNA processing, degradation, export | C1D | |||
| nup188Δ | YML103C | part of nuclear pore complex, nucleocytoplasmic transport | NUP188 | |||
| nup84Δ | YDL116W | yes | part of nuclear pore complex, nucleocytoplasmic transport | NUP107 | ||
| she4Δ | YOR035C | regulation of myosin function; asymmetric mRNA localization | STIP1 | |||
| ski2Δ | YLR398C | RNA helicase; RNA degradation | ||||
| ski8Δ | YGL213C | yes | RNA helicase; RNA degradation | |||
| sky1Δ | YMR216C | serine/arginine kinase; regulation of proteins involved in mRNA metabolism | SRPK1/2/3 | |||
| stp1Δ | YDR463W | yes | transcription factor; potential role in tRNA processing | |||
| tex1Δ | YNL253W | mRNA export | THOC3 | |||
| tif1Δ | YKR059W | translation initiation; RNA helicase | EIF4A2 | |||
| Mitochondrial and NADPH-related metabolic pathways (12/133) | ||||||
| aco2Δ | YJL200C | mitochondrial aconitase isozyme | ||||
| flx1Δ | YIL134W | mitochondrial flavin adenine dinucleotide transporter | SLC25A32 | |||
| idh2Δ | YOR136W | mitochondrial NAD(+)-dependent isocitrate dehydrogenase | IDH3A | |||
| oxa1Δ | YER154W | mitochondrial inner membrane insertase | OXA1L | |||
| rcf2Δ | YNR018W | cytochrome c oxidase subunit | ||||
| zwf1Δ | YNL241C | glucose-6-phosphate dehydrogenase | H6PD, G6PD | |||
| gor1Δ | YNL274C | mitochondrial glyoxylate reductase | GRHPR | |||
| gpd2Δ | YOL059W | NAD-dependent glycerol 3-phosphate dehydrogenase | GPD1, 1L | |||
| gph1Δ | YPR160W | glycogen phosphorylase; mobilization of glycogen | PYGL/B/M | |||
| stb5Δ | YHR178W | transcription factor; oxidative stress, stress response | ||||
| nnr2Δ | YKL151C | yes | NADHX dehydratase | CARKD | ||
| ald6Δ | YPL061W | aldehyde dehydrogenase | ALDH1A1/A2/A3, ALDH2 | |||
| Nucleotide biosynthetic pathway (7/133, P = 3.488e–5) | ||||||
| ade1Δ | YAR015W | purine nucleotide biosynthesis | PAICS | |||
| ade2Δ | YOR128C | purine nucleotide biosynthesis | ||||
| ade4Δ | YMR300C | purine nucleotide biosynthesis | PPAT | |||
| ade5, 7Δ | YGL234W | purine nucleotide biosynthesis | ||||
| ade6Δ | S000003293 | purine nucleotide biosynthesis | PFAS | |||
| ade8Δ | YDR408C | purine nucleotide biosynthesis | ||||
| bas1Δ | YKR099W | purine nucleotide biosynthesis; transcription factor | ||||
| Amino acid and other molecular biosynthetic pathways (10/133) | ||||||
| alt1Δ | YLR089C | alanine transaminase; alanine amino acid synthesis and catabolism | CCBL1/2, GPT1/2 | |||
| aro1Δ | YDR127W | synthesis of chorismate, an amino acid precursor | ||||
| cho2Δ | YGR157W | methyltransferase; phosphatidylcholine biosynthesis | ||||
| dph6Δ | YLR143W | diphthamide biosynthesis | DPH6 | |||
| elo3Δ | YLR372W | fatty acid and sphingolipid biosynthesis | ||||
| ilv1Δ | YER086W | threonine deaminase; isoleucine biosynthesis | ||||
| ino1Δ | YJL153C | inositol, inositol-containing phospholipid biosynthesis | ISYNA1 | |||
| ipk1Δ | YDR315C | yes | synthesis of phytate | IPPK | ||
| met2Δ | YNL277W | methionine biosynthesis | ||||
| met22Δ | YOL064C | methionine biosynthesis | ||||
| ER-related processes (4/133) | ||||||
| erd1Δ | YDR414C | lumenal ER protein retention | ||||
| get1Δ | YGL020C | insertion of proteins into the ER membrane | WRB | |||
| lhs1Δ | YKL073W | chaperone of the ER lumen; protein translocation and folding | ||||
| sse1Δ | YPL106C | Yes | HSP90 chaperone complex; binds unfolded proteins | HSPA4/A4L/H1 | ||
| GTPase-related proteins (7/133) | ||||||
| aim44Δ | YPL158C | cytokinesis; regulates Rho1p | ||||
| tus1Δ | YLR425W | GEF for Rho1p activity | ||||
| lte1Δ | YAL024C | similar to GDP/GTP exchange factors | RASGEF1A-C | |||
| msb3Δ | YNL293W | Rab GTPase activation; endocytosis | TBC1D, SGSM3 | |||
| gtr1Δ | YML121W | yes | part of TORC1-stimulating GTPase complex | RRAGA/B | ||
| tco89Δ | YPL180W | TORC1 subunit | ||||
| tor1Δ | YJR066W | TORC1 subunit | MTOR | |||
| DNA repair (7/133) | ||||||
| asf1Δ | YJL115W | nucleosome assembly; recovery after double-stranded DNA break repair | ASF1A/B | |||
| rad50Δ | YNL250W | yes | processing double-stranded DNA breaks | RAD50 | ||
| rad51Δ | YER095W | double-stranded DNA break repair | RAD51 | |||
| rad52Δ | YML032C | double-stranded DNA break repair | RAD52 | |||
| vps75Δ | YNL246W | histone chaperone; double-stranded DNA break repair | SET/SIP, TSPYs, FAM197Y1 | |||
| mms22Δ | YLR320W | E3 ubiquitin ligase complex involved in replication repair | ||||
| slx5Δ | YDL013W | SUMO-targed ubiquitin ligase complex; DNA repair | ||||
| Serine/threonine and serine modifiers (8/133) | ||||||
| fus3Δ | YBL016W | mitogen-activated serine/threonine protein kinase | MAPK1,3,4,5,6 or NLK | |||
| ptk2Δ | YJR059W | serine/threonine protein kinase; regulation of ion transport | TSSKs | |||
| yck3Δ | YER123W | vacuolar membrane serine/threonine kinase; vacuole fusion | ||||
| pph21Δ | YDL134C | catalytic subunit of protein phosphatase 2a (serine/threonine phosphatase); mitosis | ||||
| ppm1Δ | YDR435C | Yes | methyltransferase; methylates the C terminus of Pph21p | LCMT1 | ||
| rts1Δ | YOR014W | regulatory subunit of protein phosphatase 2A | PPP2R5C/D | |||
| kex2Δ | YNL238W | calcium-dependent serine protease | ||||
| prb1Δ | YEL060C | vacuolar serine protease | ||||
| Acetyltransferases (3/133) | ||||||
| eaf6Δ | YJR082C | part of acetyltransferase complex; histone acetylation | MEAF6 | |||
| hpa3Δ | YEL066W | D-Amino acid N-acetyltransferase; histone acetylation | ||||
| mak10Δ | YEL053C | NatC N-terminal acetyltransferase | NAA35 | |||
| Other (8/133) | ||||||
| alf1Δ | YNL148C | yes | alpha-tubulin folding; microtubule maintenance | TBCB, CLIP3/4 | ||
| atx1Δ | YNL259C | cytosolic copper metallochaperone | ATOX1 | |||
| cdc50Δ | YCR094W | endosomal protein; involved with Golgi membrane trafficking | TMEM30A/B/C | |||
| clb2Δ | YPR119W | yes | cell cycle progression | CNTD2 | ||
| fcy22Δ | YER060W-A | purine-cytosine permease | ||||
| fen2Δ | YCR028C | H+-pantothenate symporterH | ||||
| sho1Δ | YER118C | transmembrane osmosensor for filamentous growth | ||||
| vps64Δ | YDR200C | yes | cytoplasm to vacuole targeting of proteins | TRAF3IP3, SLMAP, CEP170/B, CCDC136 | ||
| Uncharacterized proteins (13/133) | ||||||
| brp1Δ | YGL007W | protein of unknown function | ||||
| fyv1Δ | YDR024W | dubious ORF | ||||
| fyv6Δ | YNL133C | protein of unknown function | ||||
| gds1Δ | YOR355W | protein of unknown function | ||||
| hhy1Δ | YEL059W | dubious ORF | ||||
| irc14Δ | YOR135C | dubious ORF | ||||
| mtc7Δ | YEL033W | protein of unknown function | ||||
| rtc4Δ | YNL254C | protein of unknown function | ||||
| sdd1Δ | YEL057C | protein of unknown function | ||||
| ydr417cΔ | YDR417C | yes | dubious ORF | |||
| ygl165cΔ | YGL165C | yes | dubious ORF | |||
| ynl198cΔ | YNL198C | yes | dubious ORF | |||
| ynr005cΔ | YNR005C | yes | dubious ORF | |||