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. 2018 Apr;20(4):228–235. doi: 10.1016/j.micinf.2017.12.011

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© 2017 The Author(s)

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Fig. 4 — Recognition of recombinant TBCG_03312 C-terminus by sera from TB patients from Peru, Vietnam, and South Africa. Sera were tested by conventional ELISA using the purified C-terminus of TBCG_03312 [inset; Lane 1, IPTG-induced E. coli culture; Lane 2, flow through of IPTG-induced E. coli culture; Lane 3, wash; Lane 4, purified TBCG_03312 C-terminus protein (arrow)]. Dotted red line in the graph represents the mean of the OD obtained for six normal human serum (NHS) samples (Methods) plus 3 SD of the mean. S-pos, smear positive; C-pos, culture positive.