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. 2018 May 8;6:e4737. doi: 10.7717/peerj.4737

Figure 3. VB-1 promotes Wnt/β-catenin signaling in human DPCs.

Figure 3

(A) Concentration-dependent effects (0–10 µM) of VB-1 on dkk1 mRNA expression in human DPCs cultured for 24 h. (B) Concentration-dependent effects (0–10 µM) of VB-1 on tgf-β mRNA expression in human DPCs cultured for 24 h. (C) Immunoblotting analysis of active β-Catenin expression in hDPCs treated with VB-1 (0–10 µM) for 24 h. (D) Quantification of active β-Catenin protein expression. (E) Real-time PCR analysis for gene expression of axin2 in hDPCs treated with VB-1 (0–10 µM) for 24 h. (F) Immunoblotting analysis of AXIN2 expression in hDPCs treated with VB-1 (0–10 µM) for 24 h. (G) Quantification of AXIN2 protein expression. Experiments were carried out in triplicates. The typical blot was presented and quantification of three independent experiments is shown for C and F. Data are reported as mean+SEM. Student’s t-test was used to compare data. *P < 0.05, **P < 0.01, “ns” indicates no significant difference.