Table 1.

Primers and probes designed for this study

Genotype specificity	Primer type	Sequence	Genome positiona
PCR
Gt1a	OS	CAT ATA ACG GGY CAY CGC ATG G	1275‐1296
	OAS	TGG TTY GGY TGY ACY TGG ATG AA	2008‐1986
	IS	ATG ATR ATG AAC TGG TCY CCY AC	1305‐1327
	IAS	TYG TCC TYA AYA ACA CYA GRC C	1972‐1951
Gt3	OS	TTY CTY GTG GGR CAA GCC TTC A	1203‐1224
	OAS	CCT YTW CTG CCC CAC YGA CTG	2143‐2123
	IS	TTY AGA CCY CGY CGC CAT CA	1227‐1246
	IAS	CAG AYG TGT TCY TGC TGR AGT C	1993‐1972
Pan‐genotypic	S	GC NTG GGA YAT GAT GAT GAA YTG	1296‐1318
	AS	GDG SGT ART GCC ARC ART ANG	1812‐1792
Real‐time PCR
Gt1a	S	CTG TCG AGC CGC AGG GCT C	8507‐8525
	AS	GCT CCA AGT CGT AYT CTG GYT GBG	8686‐8662
	Probe	FAMTM‐CCT CCG TGA AGG CTC TCA GGY TCG CYG CG‐MGB	8625‐8597
Gt3	S	GGA ACC CGG ACT TYC TYG TCT G	8527‐8548
	AS	CTC AAG GTC RTA GGT RGG CTG YGG	8684‐8661
	Probe	FAMTM‐CGA CGC CRT CAC TCT CRG CCA CCA CRA CYA G‐MGB	8589‐8559

O, outer; I, inner; S, sense; AS, antisense.

^aGenome position is relative to the HCV strain H77 (Kuiken et al., 2006).