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editorial
. 2018 Mar 24;285(8):1403–1418. doi: 10.1111/febs.14426

Figure 6.

Figure 6

BFL1 overexpression protects premalignant immature B cells from MYC‐induced apoptosis. (A) White blood cell counts from 2–week‐old premalignant Eμ‐MYC, Eμ‐MYC/Vav‐BFL1 (L1 and L3 pooled), and Eμ‐MYC/Vav‐BCLX (line A) mice were assessed. (B) Percentage of B220+ B lymphoid cells in the bone marrow was analysed by flow cytometry (left bar). B220+ cells were further discriminated into CD19−CD4+ cells (right bar). (C) Total bone marrow was cultured for 30 h and the abundance of living (Annexin V−) B220+IgM− immature B lymphoid cells was assessed by flow cytometry. (D) Abundance of total B220+ B lymphoid cells in the spleen was analysed by flow cytometry (left graph). B220+ cells were further discriminated into CD19+IgM− (middle graph) and CD19−CD4+ cells (right graph). (E) Total splenocytes were cultured for 30 h and abundance of living (Annexin V−) B220+IgM− immature B lymphoid cells was assessed by flow cytometry. Statistical analysis was performed by using a one‐way ANOVA with Dunnett's multiple comparison test compared to Eμ‐MYC control. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001; n ≥ 3 ± SD.