Figure 1.

Phylogeny of 190 genomes and cry toxicity in the Bacillus cereus species complex. (a) The phylogenetic tree was reconstructed using gene‐by‐gene concatenated alignments of 2,274 core genes, and an approximation of the maximum‐likelihood algorithm implemented in RAxML. The scale represents the number of substitutions per site. Clades previously defined by multilocus sequence typing are specified in bold. cry endotoxin genes were identified in the genomes with BtToxin_scanner software and are indicated as present (green) or absent (white) for each genome. Isolates from the Bacillus anthracis clade are shown in pink. Numbers next to the tip of branches on the tree indicate sequence types from the B. cereus pubMLST database (https://pubmlst.org/bcereus/). (b) Inferred invertebrate host range of B. cereus group isolates based on known toxicity spectra of cry genes present in genomes. Host range allocations are detailed in Table S1 and based on data in van Frankenhuyzen (2009) and sources within the Cry nomenclature database (Crickmore et al., 2016)) [Colour figure can be viewed at http://www.wileyonlinelibrary.com/]