Figure 4.

In vitro recall response to OVA for DLN cells from WT, SATB1cKO_V and SATB1cKO_L mice. (a) Mice were immunized with OVA in CFA. DLN cells were prepared 10 or 28 days after immunization and incubated with OVA at the indicated doses; proliferation was assessed on Day 3. Production of IL‐2 (Day 1), IL‐17 (Day 3), and IFN‐γ (Day 3) was determined by ELISA. (b) DLN cells were prepared from naïve WT and SATB1cKO_L mice or from mice on Day 10 after immunization, as described in the legend of Figure 4(a). DLN cells were incubated with OVA, after which intracellular expression of IL‐17 and IFN‐γ was assessed. The numbers in the light quadrants represent the percentages of all cells. * P < 0.05. One representative result of three independent experiments is shown. [Color figure can be viewed at http://wileyonlinelibrary.com]