Figure 7.

Encephalitogenic gene expression in Th17 cells not expressing SATB1. To prepare Th17 cells, DLN cells (CD45.2) from MOG immunized SATB1cKOe mice were skewed using MOG peptide and IL‐23 and then transferred into recipient mice (CD45.1). Some of these mice were treated with tamoxifen on Days 1, 3, and 5 after the transfer. On Day 7, CD4⁺ CD45.2⁺ transferred donor T cells were sorted from the spleen and lymph nodes of recipient mice using a FACS Aria III cell sorter. (a) Quantitative PCR was used to measure mRNA expression of Il17A, Ifng, Rorc, Il23r, Satb1 and Foxp3 in donor CD4 T cells. *Values differ significantly from those in control cells (SATB1‐sufficient Th17 cells) under the same conditions (P < 0.05). Experiments were conducted three times, with essentially similar results. (b) CD45.2⁺ CD4⁺ Th17 cells from SATB1‐deficient mice (with tamoxifen) or SATB1‐sufficient mice (without tamoxifen), or control CD4 T cells from naïve C57BL/6 mice were assessed for intracellular IL‐17 or Foxp3 expression. The numbers in the squares are the percentages of CD45.2⁺ CD4⁺ cells.