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Validation of AlphaLISA DUB assay. (a) Scheme showing the AlphaLISA cell lysate DUB assay procedure. (b) Dose–response curve showing the inhibition of UCHL1 by LDN-57444, ML323, and Ubiquitin-Br using AlphaLISA cell lysate DUB assay. (c) Inhibition of HA-UCHL1 by LDN-57444 and ML323 visualized by Western blotting using anti-HA antibody.
