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. Author manuscript; available in PMC: 2018 Sep 1.
Published in final edited form as: J Thorac Cardiovasc Surg. 2017 May 24;154(3):955–963. doi: 10.1016/j.jtcvs.2017.04.081

Figure 1.

Figure 1

Nanovolume capillary electrophoresis-based protein analysis was performed on cultured mesenchymal stem cells (MSCs) and transdifferentiated smooth muscle cells (SMCs) (MSC, n=3; SMC, n=3). (A) Electropherogram and system software generated peak area and molecular weight data linear analysis for alpha smooth muscle actin (SMA), SM22 alpha, and caldesmon between MSCs and SMCs. Green indicates SMC; blue, MSC. (B) Representative immunoblots for alpha SMA, SM22 alpha, and caldesmon between MSC and SMC. (C) Transdifferentiated SMCs demonstrated higher protein concentrations of alpha SMA, SM22 alpha, and caldesmon than MSCs. The intensity level of detected protein bands was standardized by dividing by the intensity level of vinculin.