Figure 1.
Manhattan plot of the genome‐wide association analysis. Analysis of 36 cases induced by sulfasalazine vs. all controls with 9,380,034 SNPs after imputation, adjusted by sex and genetic principal components 1–4. The red line shows the threshold for genome‐wide significance of 5 × 10−8. The top SNP was rs9266634 marked in green is located in an intergenic region close to HLA‐B on chromosome 6. There was also a significant association with an intergenic insertion/deletion on chromosome 20, rs202233001, marked in green. The closest protein coding gene to this variant is the transcription factor gene Paired Box 1 (PAX1). After adjustment for rs9266634, the intronic SNP rs111876221 in the gene serine incorporator 5 (SERINC5) on chromosome 5, reached genome‐wide significance. After adjustment for both rs9266634 and rs111876221, rs113887891 in a nonprotein coding RNA gene (LINC01762) on chromosome 1 was significant on a genome‐wide level. After adjusting also for this SNP, the intron variant rs12082628 in the gene cholinergic receptor, muscarinic 3 (CHRM3) on chromosome 1 gave a significant result. SNP = single nucleotide polymorphism, HLA = human leukocyte antigen, RNA = ribonucleic acid.