Fig. 1. NBD–UBI enables live confocal imaging of bacteria. UBI-2 demonstrates higher signal to noise for bacterial labelling over UBI-1 when imaged via confocal microscopy. Upper three panels showing UBI-1 (10 μM) with P. aeruginosa; lower panel demonstrates UBI-2 (10 μM) with P. aeruginosa. Panel insets show cross section of a single bacterium with a plot profile (right of main panel) across the FAM/NBD image in green and the counterstain in red, demonstrating fluorescence of UBI-2 but not UBI-1 across the bacteria scale bar = 5 μm.
