Fig. 4. Reduction of fluorescent intensity of P. aeruginosa labelled with UBI-3 in the presence of human ARDS BALF. Representative images of P. aeruginosa imaged with UBI-3 (10 μM) by confocal microscopy in the presence of phosphate buffered saline (PBS) or ARDS BALF. Quantification of fluorescence intensity demonstrating significantly reduced intensity when compared to PBS (bars represent mean (±SEM), n = 3 for PBS and n = 5 for ARDS, *** = p < 0.001, scale bar = 10 μm).
