Figure 2.

(A) Images showing the wound‐healing assay for cellular migration in human prostate cancer cell line DU145 and PC3 pretreated for 24 h with 5 ng/mL of TGFβ or TGFβ and 50 μM 2‐APB. Images were taken immediately after the wound scratch (0 h) and after 24 h. The pictures are representative of four separate experiments. (B) Western blotting showing the expression of Vimentin, N‐cadherin, E‐cadherin, and loading control β‐actin in DU145 and PC3 cells. (C) DU145 cells were treated with 5 ng/mL TGFβ for 24 h before being trypsinized, re‐suspended in serum free media with or without 50 μM 2‐APB, and placed in transwell inserts for 6 h. Four random fields at 10× were counted from four separate experiments indicating total cells migrated ± SEM. * indicates significance (P < 0.05). (D) DU145 cells were treated with TGFβ for various times as included in the figure, lysed proteins were subjected to SDS‐PAGE followed by Western blotting with respective antibodies. (E) IV curves of TRPM7 current under various conditions (control, TGFβ or TGFβ and 2‐APB.) and quantitation (8‐10 recordings) of current intensity at ±100 mV is shown in (F). * indicates significance (P < 0.05)