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. 2018 Feb 27;285(7):1237–1250. doi: 10.1111/febs.14402

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© 2018 The Authors. The FEBS Journal published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Zinc stoichiometry of the two ZnT8 CTD variants. Fraction of the maximum Zn²⁺ content of 10 μm ZnT8cR (teal diamonds) and ZnT8cW (red circles) following incubation with 0–10 molar equivalents of Zn²⁺ and subsequent gel filtration to remove unbound Zn²⁺. Protein concentration was determined spectrophotometrically (Materials and methods). The intersection points in the titration data indicate that ZnT8cR binds Zn²⁺ with a stoichiometry of 2.6 ± 0.4 per monomer, whereas ZnT8cW binds 3.2 ± 0.5 per monomer. The difference between the two variants is not statistically significant (n = 3 for both variants, P = 0.156).