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. 2018 Jan 26;74(Pt 2):105–112. doi: 10.1107/S2053230X18000262

Table 1. Macromolecule-production information.

Source organism P. gingivalis strain W83 (BAA-308D-5)
DNA source gDNA
Forward primer 5′-GAA TAA GCA TAT GAA TAG AAT TTG CGA ATT ATT GGG T-3′
Reverse primer 5′-AGA TGG ATC CTC ATA TCT CAG TGG G-3′
Cloning vector pET-15b
Expression vector pET-15b
Expression host E. coli BL21 (DE3)
Complete amino-acid sequence of the construct produced MGSSHHHHHHSSGLVPRGSHMNRICELLGIEHPIISGGMVWCSGWKLASAVSNCGGLGLIGAGSMHPDNLEHHIRSCKAATDKPFGVNVPLLYPEMDKIMEIIMREHVPVVVTSAGSPKVWTAKLKAAGSKVIHVVSSATFARKSEAAGVDAIVAEGFEAGGHNGREETTTLCLIPEVVDAVNIPVVAAGGIASGRAVAAALALGADAVQVGTRFALSEESSAHEDFKAHCRRSVEGDTMLSLKAVSPTRLLKNKFYQDVFAAEQRGASVEELRELLGRGRAKQGIFEGDLHEGELEIGQAVSQISHAETVAEIMVDLVDGYKRSLAGMPTEI