Table 1. Macromolecule-production information.
Source organism | P. gingivalis strain W83 (BAA-308D-5) |
DNA source | gDNA |
Forward primer | 5′-GAA TAA GCA TAT GAA TAG AAT TTG CGA ATT ATT GGG T-3′ |
Reverse primer | 5′-AGA TGG ATC CTC ATA TCT CAG TGG G-3′ |
Cloning vector | pET-15b |
Expression vector | pET-15b |
Expression host | E. coli BL21 (DE3) |
Complete amino-acid sequence of the construct produced | MGSSHHHHHHSSGLVPRGSHMNRICELLGIEHPIISGGMVWCSGWKLASAVSNCGGLGLIGAGSMHPDNLEHHIRSCKAATDKPFGVNVPLLYPEMDKIMEIIMREHVPVVVTSAGSPKVWTAKLKAAGSKVIHVVSSATFARKSEAAGVDAIVAEGFEAGGHNGREETTTLCLIPEVVDAVNIPVVAAGGIASGRAVAAALALGADAVQVGTRFALSEESSAHEDFKAHCRRSVEGDTMLSLKAVSPTRLLKNKFYQDVFAAEQRGASVEELRELLGRGRAKQGIFEGDLHEGELEIGQAVSQISHAETVAEIMVDLVDGYKRSLAGMPTEI |