| Method |
Vapor diffusion, hanging drop |
| Plate type |
24-well VDX plate with sealant (Hampton Research) |
| Temperature (K) |
291 |
| Protein concentration (mg ml−1) |
5 |
| Buffer composition of protein solution |
Initial: 10 mM MOPS pH 7.5, 100 mM NH4Cl, 1.4 mM NADPH, 5 mM DTT |
| Microseeding: 0.15 M sodium citrate pH 5.6, 10%(w/v) PEG 3350 |
| Composition of reservoir solution |
Initial crystal: 0.15 M sodium citrate pH 5.6, 10% PEG 3350 |
| Microseeding: 0.2 M sodium formate pH 7.0, 20% PEG 3350 |
| Volume and ratio of drop |
3 µl protein stock:3 µl Index condition No. 90:1 µl seed stock (seed stock from 10% PEG 3350, 0.15 M sodium citrate pH 5.6) |
| Volume of reservoir (µl) |
500 |
