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. 2018 Feb 26;74(Pt 3):128–134. doi: 10.1107/S2053230X18000857

Table 2. Crystallization.

Method Hanging-drop vapor diffusion
Plate type 24-well plates from Hampton Research
Temperature (K) 293
Protein concentration (mg ml−1) 15
Buffer composition of protein solution 50 mM Tris pH 8.0, 150 mM NaCl, 5 mM β-mercaptoethanol
Composition of reservoir solution 0.2 M sodium acetate, 20% PEG 3350
Volume and ratio of drop 3 µl (1:1)
Volume of reservoir (µl) 500