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. 2018 Feb 26;74(Pt 3):156–160. doi: 10.1107/S2053230X18002108

Table 2. Crystallization.

Method Hanging-drop vapour diffusion
Plate type VDXm plate
Temperature (K) 277
Protein concentration (mg ml−1) 11.4
Buffer composition of protein solution 20 mM Tris–HCl pH 7.5, 50 mM NaCl, 3 mM DTT
Composition of reservoir solution 12%(w/v) polyethylene glycol 3350, 200 mM ammonium sulfate, 100 mM sodium citrate buffer pH 6.2, 20 mM magnesium chloride
Ratio of drop 1:1
Volume of reservoir (ml) 0.4