Table 2. Crystallization.
Method | Hanging-drop vapour diffusion |
Plate type | VDXm plate |
Temperature (K) | 277 |
Protein concentration (mg ml−1) | 11.4 |
Buffer composition of protein solution | 20 mM Tris–HCl pH 7.5, 50 mM NaCl, 3 mM DTT |
Composition of reservoir solution | 12%(w/v) polyethylene glycol 3350, 200 mM ammonium sulfate, 100 mM sodium citrate buffer pH 6.2, 20 mM magnesium chloride |
Ratio of drop | 1:1 |
Volume of reservoir (ml) | 0.4 |