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| Method | Hanging-drop vapour diffusion |
| Plate type | Linbro 24-well plate |
| Temperature (K) | 277 |
| Protein concentration (mg ml−1) | 20 |
| Buffer composition of protein solution | 20 mM Tris–HCl pH 8.0, 250 mM NaCl, 5% glycerol |
| Composition of reservoir solution | 100 mM Tris–HCl buffer pH 8.5, 25% PEG 3350 |
| Volume and ratio of drop | 2 µl; 1:1 |
| Volume of reservoir (µl) | 100 |
