Hepatic gene expression in free fed vs 18 hour fasted mice. A) Foxo1, B) Pck1, C) G6pc, D) Irs-2, E) Srebflc, F) Fasn, G) Insig1, H) Ppargc1a. Black bars represent IR het only, white bars IR/PPP2R2A double hets. N=8 biological replicates, data is represented as mean ± SD, statistical analysis 2 way ANOVA compared to IR het only fasted * p<0.05, **p<0.01, ***p<0.001.