Table 1.

Knockdown of Ppp2r2a reduced PPP2R2A and AKT protein levels, insulin stimulated AKT phosphorylation

	Cell line	Relative protein levels PPP2R2A (%)	P-value	Relative protein levels AKT (%)	P-value		Phosphorylation normalized to tubulin		P-value saline vs insulin	P-value control saline vs saline	Direction of effect on basal P	P-value control insulin vs insulin	Direction for stimulation
							saline	insulin
control	Hepa	100±24		100±24.5		pAKT(thr308)	2.31±0.76	10.78±1.3	<0.0001
siRNA1	1-6	17.55±3.9	<0.0001	42.68±10.6	<0.0001		3.51±0.51	6.44±1.78	0.003	0.009	↑	0.0007	↓
siRNA3		17.8±5.6	<0.0001	34.90±12.3	<0.0001		4.35±0.51	4.84±0.49	ns	0.0002	↑	<0.0001	↓
control	Hepa					pAKT(ser473)	7.23±0.89	27.74±3.49	<0.0001
siRNA1	1-6						4.83±1.35	17.31±4.70	<0.0001	0.004	↓	0.001	↓
siRNA3							8.73±1.04	17.81±1.94	<0.0001	0.02	↑	0.0001	↓
control	C2C12	100±37.5		100±30.3		pAKT(thr308)	1.19±0.25	8.26±2.58	<0.0001
siRNA1		27.33±6.7	<0.0001	82.19±6.8	0.06		4.45±0.63	6.03±1.31	0.02	<0.0001	↑	ns	↓
siRNA3		35.78±20.3	<0.0001	73.22±18.3	0.01		4.10±0.87	5.06±2.09	ns	<0.0001	↑	0.03	↓
control	C2C12					pAKT(ser473)	5.06±2.11	36.83±11.8	<0.0001
siRNA1							7.54±1.44	25.40±2.94	<0.0001	0.03	↑	0.04	↓
siRNA3							8.25±0.87	19.24±6.99	0.003	0.006	↑	0.01	↓
control	3T3-L1	100±18.4		104±14.6		pAKT(thr308)	0.53±0.19	1.98±0.26	<0.0001
siRNA1		64.63±17.3	<0.0001	77.08±12.3	<0.0001		1.00±0.12	1.62±0.91	ns	0.0006	↑	ns
siRNA3		65.26±11.4	<0.0001	72.31±8.1	<0.0001		0.79±0.27	1.50±0.86	ns	ns		ns
control	3T3-L1					pAKT(ser473)	0.61±0.03	2.45±0.39	<0.0001
siRNA1							1.99±0.51	2.45±0.38	ns	<0.0001	↑	ns
siRNA3							1.10±0.22	2.25±0.48	0.0003	0.0003	↑	ns