Table 2.

Knockdown of Ppp2r2a reduced downstream insulin AKT signaling.

	Cell line	Relative protein levels GSK-3β	P-value	Relative protein levels p70S6K	P-value		% Phosphorylation		P-value saline vs insulin	P-value control saline vs saline	Direction of effect on basal P	P-value control insulin vs insulin	Direction for stimulation
							saline	insulin
control	Hepa	99±6.8				pGSK-3β	53.03±7.12	107±10.80	<0.0001
siRNA1	1-6	113.92±12.9	ns				65.27±7.79	61.95±9.16	ns	0.01	↑	<0.0001	↓
siRNA3		109.13±13.6	ns				69.68±3.29	58.09±5.52	ns	0.0004	↑	<0.0001	↓
control	Hepa			100±5.5		pp70S6K	4.24±0.35	10.22±0.73	<0.0001
siRNA1	1-6			109.13±13.6	ns		10.58±1.19	10.62±1.34	ns	<0.0001	↑↑	ns
siRNA3				90.61±17.6	ns		9.44±1.79	10.49±1.74	ns	<0.0001		ns
control	C2C12	100±12.45				pGSK-3β	59.16±15.65	105.54±11.2	<0.0001
siRNA1		113.94±8.9	0.04				45.76±3.16	56.16±19.59	ns	ns		0.0003	↓
siRNA3		151.30±32.9	0.02				58.45±14.03	69.13±10.40	ns	ns		0.0001	↓
control	C2C12			100±20.5		pp70S6K	6.37±2.07	12.36±2.33	0.0008
siRNA1				129.41±16.2	0.02		9.71±0.70	10.51±0.51	0.04	0.003	↑	ns
siRNA3				99.99±6.9	0.01		10.76±0.85	9.92±1.30	ns	0.0007	↑	ns
control	3T3-L1	100±29.9				pGSK-3β	44.47±5.75	89.48±2.90	<0.0001
siRNA1		101.15±33.7	ns				72.44±7.40	92.36±10.89	0.004	<0.0001	↑	ns
siRNA3		105.03±27.9	ns				65.9±6.24	86.16±13.43	0.007	0.0001	↑	ns
control	3T3-L1			99±19.39		pp70S6K	4.74±0.81	8.23±0.42	<0.0001
siRNA1				105.39±28.5	ns		4.91±0.82	5.06±0.41	ns	ns		<0.0001	↓
siRNA3				99.99±6.9	ns		6.68±0.71	7.85±0.638	0.01	0.001	↑	ns