Figure 1.

Evaluation of crude extract house dust mite (HDM) and naturally purified Der p1 and 2. A, Purified proteins were separated by sodium dodecyl sulfate‐polyacrylamide gel electrophoresis SDS‐PAGE in four different conditions: 1, zero hour; 2, 46‐h incubation at 4°C; 3, 46‐h incubation at room temperature; 4, 46‐h incubation at 37°C. On the left, the marker (M) is consistent throughout all gels. B, left: Analysis of purified Der p1 by PAA gel electrophoresis identifying three smaller fragments, sized 25, 17, and 14 kDa. B, right: Protein BLAST against full‐length Der p 1 of peptides identified by Maldi‐TOF from purified bands a, b, and c