Skip to main content
. 2018 May 8;11:935–945. doi: 10.2147/JPR.S159458

Figure 2.

Figure 2

miR-34a can bind and regulate XIST.

Notes: (A) Relative luminescence after transfecting HEK293 cells with XIST pmirGLO plasmid with 5 kb fragment from 5′XIST cloned downstream of the firefly luciferase gene, and precursor miR-34a or control miRNA (n=5). (B) and (C) Relative expression of Xist/XIST in mouse J774A.1 and human SH-SY5Y cells, respectively, after miR-34a transfection. Expression of Xist/XIST normalized to Gapdh/GAPDH. Student’s t-test used for statistical analysis *p<0.05, ***p<0.001. Data represent mean ± SEM.

Abbreviations: SEM, standard error of the mean; XIST, X-inactive-specific transcript.