Figure 4.

Upregulation of Xist and downregulation of miR-34a in blood samples from CFA model of inflammatory pain.

Notes: (A) CFA-induced mechanical hypersensitivity assessed using von Frey filaments in female C57BL/6 mice (n=3–4). Paw withdrawal threshold dropped significantly by 4 h (acute inflammatory phase) and remained low at 14 days (chronic inflammatory phase). (B) Relative expression of Xist in female C57BL/6 mice injected with CFA or saline into the hind paw (n=3–4). Blood samples collected at 4 h and 14 days were used to determine the expression of Xist during acute and chronic inflammatory phases respectively. (C) Relative expression of miR-34a in blood under acute and chronic inflammation in female C57BL/6 mice injected with CFA or saline (n=3–4). One-way ANOVA and Student’s t-test were used for statistical analysis except for mice mechanical hypersensitivity in which Mann–Whitney U test was used *p<0.05, **p<0.01, ***p<0.001, data represent mean ± SEM.

Abbreviations: ANOVA, analysis of variance; CFA, complete Freund’s adjuvant; SEM, standard error of the mean; Xist, X-inactive-specific transcript.