Fig 2. Increased expression of adeA following pentamidine stress is dependent on the presence of AdeRS in ATCC 17978.

Transcriptional levels of adeA (ACX60_09125) from WT and ΔadeRS were determined by qRT-PCR after 30 min shock with 7.8 mg/L of pentamidine (Pent) (0.5 × MIC of ΔadeRS) and corrected to untreated cells (UT) after normalization to 16S. Bars represent the mean fold change (Log₂) of three biological replicates undertaken in triplicate, and error bars represent ± SEM. Statistical analyses were performed by Student’s t-test, two-tailed, unpaired; ** = P < 0.01 and *** = P < 0.001.