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. 2018 May 11;13(5):e0197412. doi: 10.1371/journal.pone.0197412

Fig 3. Resistance to pentamidine is modulated by carbon sources available in the growth medium.

Fig 3

Ten-fold serial dilutions of A. baumannii ATCC 17978 (WT), ΔadeRS (ΔRS) and ΔadeAB (ΔAB) cells were used to compare the concentration of pentamidine that inhibits growth in different media, Mueller-Hinton agar (MHA) was used as a comparative control. Images display serial 1:10 dilutions after overnight incubation at 37°C, where DF is abbreviated for dilution factor and N represents undiluted cells. Strains were grown in the absence of pentamidine (UT) or presence of 32, 64, 128, 256 and 512 mg/L of pentamidine (P32, P64, P128, P256, and P512, respectively). Carbon sources tested in M9 minimal medium were used at a final concentration of 0.4% (w/v). ND, not done due to precipitation of pentamidine once added into the molten medium. Figures are representative examples of results obtained.