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. 2018 May 11;13(5):e0197046. doi: 10.1371/journal.pone.0197046

Fig 3. In vivo transplantation of 14TF iENDO cells under the kidney capsule forms endodermal tumors but that differentiate into hepatoblasts and pancreatic endocrine like cells.

Fig 3

A) Schematic representation of transplantation of undifferentiated iENDO cells under the kidney capsule of RAG2-/- γc-/- mice. B) Morphology of control kidney (half portion shown) and iENDO cell grafted kidney after three weeks (N = 3) and after three months (N = 12). C) H&E stained sections of iENDO graft (10x and 20x magnification) 3 weeks after transplantation (scale bar 50μm) (N = 3). D) H&E stained sections of iENDO graft (20x magnification) 3 months after transplantation (mouse 1 and mouse 2)(scale bar 100μm) Representative for (N = 12) mice. E) Human albumin quantification by ELISA from iENDO cell grafted mice 3 weeks (N = 3) and 3 months (N = 12) after transplantation, untransplanted mouse serum and human blood serum. F) Human C-peptide by ELISA (untransplanted mouse serum, adult human blood serum and 14TF iENDO grafted mouse 3 months after transplantation (N = 12). *p<0.05, **p<0.01 and ***p<0.001 determined by unpaired 2-tailed Student’s t-test. ND- Not determined, NS- Not significant.