Table 2. Summary of knock-in lines generated using csRNP delivery.
Table summarising knock-in lines derived from mouse NS and GBM-model cells. The percentage bi-allelic knock-in among all V5-positive clones, as confirmed by PCR genotyping, is listed in the last column.
Table 2—source data 1. Raw data for V5 ICC and PCR genotyping of clonal knock-in lines.
elife-35069-table2-data1.xlsx (27.6KB, xlsx)
DOI: 10.7554/eLife.35069.012
| Cell type | Gene | Colonies picked | V5 positive (ICC) | Correctly targeted (PCR) | Bi-allelic clones by PCR (% of total V5 positive) |
|---|---|---|---|---|---|
| Mouse NS cells | Sox2 | 89 | 13 | 11 | 4 (30.8%) |
| Sox3 | 55 | 8 | 6 | 5 (62.5%) | |
| Mouse GBM-model cells | Sox2 | 242 | 14 | 14 | 1 (7.1%) |
| Sox3 | 96 | 19 | 18 | 5 (26.3%) | |
| Sox8 | 96 | 7 | 6 | 4 (57.1%) | |
| Sox9 | 469 | 46 | 41 | 7 (15.2%) | |
| Foxk2 | 96 | 2 | 2 | 1 (50%) |