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. Author manuscript; available in PMC: 2019 Feb 1.
Published in final edited form as: J Bone Miner Res. 2017 Oct 9;33(2):338–355. doi: 10.1002/jbmr.3293

Figure 6. Inhibition of CA9 and CA12 in rat NP cells leads to reduced OCR without affecting mitochondrial respiration or functional activity.

Figure 6

A–C) OCR was reduced in rat NP cells treated with MZA (500 nM) (A), AZA (100 nM, 500 nM) (B) and U-104 (500 nM, 2 μM) (C) before and after treatment with antimycin A. D) Treatment of rat NP cells with MZA, AZA, and U-104 significantly decreased average OCR. E) Treatment of rat NP cells with MZA, AZA, and U-104 has no effect on mitochondrial OCR. F) Rat NP cells treated with MZA, AZA, and U-104 showed no change in their mitochondrial oxidoreductase activity, implying preservation of mitochondrial function. All quantitative data is represented as mean ± SE, n=3 independent biological replicates with 4 technical replicates per biological replicate, p<0.05.