Skip to main content
. Author manuscript; available in PMC: 2019 Feb 1.
Published in final edited form as: J Bone Miner Res. 2017 Oct 9;33(2):338–355. doi: 10.1002/jbmr.3293

Figure 9. Inhibition of CA9/12 causes a significant decrease in intracellular pH by blocking the generation of recycled bicarbonate.

Figure 9

A) Schematic of extracellular HCO3− generation and its intracellular recycling. B) Intracellular pH significantly decreased after CA inhibition (n=3, 3-4 technical replicates each). C) qRT-PCR results showing expression of bicarbonate transporters in rat NP cells. D) Intracellular pH significantly decreased after SITS treatment for 18 hours under hypoxia (n=4, 3 technical replicates each). E) Treatment of rat NP cells with MZA (500 nM), AZA (500 nM), and U-104 (2 μM) for 48 and 96 hours in hypoxia significantly decreased cell viability. Data is represented as mean ± SE, n ≥3 biological replicates, 3-6 technical replicates per biological replicate, * p<0.05, ** p<0.01, *** p<0.001. (F) Schematic of the proposed model of intracellular pH regulation through HCO3− recycling by the HIF-1α-Carbonic anhydrase axis in NP cells.